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Channel Rhodopsins01:11

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Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
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An Enzyme-Catalyzed Optical Fiber Optofluidic Laser Sensor for Cholesterol Detection Using Rhodamine B Hydrazide.

Hongrui Zhang1, Ya-Nan Zhang1,2,3, Like Li1

  • 1College of Information Science and Engineering, Northeastern University, Shenyang 110819, China.

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This study introduces a novel rhodamine-based method for sensitive cholesterol detection. The developed optical fiber optofluidic laser sensor offers rapid, cost-effective, and selective cholesterol quantification, even in human serum.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Cholesterol determination is crucial for diagnosing cardiovascular diseases.
  • Existing methods for cholesterol measurement can be time-consuming and costly.
  • Development of sensitive and rapid cholesterol detection platforms is needed.

Purpose of the Study:

  • To develop a sensitive and rapid method for cholesterol determination using rhodamine derivatives.
  • To utilize optical fiber optofluidic lasers (FOFLs) for enhanced fluorescence detection.
  • To validate the sensor's performance for cholesterol measurement in biological samples.

Main Methods:

  • Employing rhodamine B hydrazide (RBH) as a chromogenic substrate for hydrogen peroxide (H2O2) detection.
  • Utilizing a peroxide-mimicking enzyme catalyst and an oxidase system for indirect cholesterol quantification.
  • Integrating the reaction with FOFLs for sensitive fluorescence-based detection.

Main Results:

  • Achieved a dynamic range of three orders of magnitude for H2O2 determination with a limit of detection (LOD) of 1.63 μM.
  • Developed a cholesterol sensor with a linear range of 2.55-652.67 μM and an LOD of 2.55 μM.
  • Demonstrated good selectivity and initial feasibility for cholesterol determination in human serum within a 3.5-minute assay time.

Conclusions:

  • The proposed rhodamine-based method combined with FOFLs provides a sensitive, rapid, and cost-effective platform for cholesterol detection.
  • The sensor exhibits excellent performance characteristics, including a wide dynamic range and low LOD.
  • This approach holds promise for clinical applications in cholesterol monitoring.