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Related Concept Videos

Role of Septins01:02

Role of Septins

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Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
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Septins01:19

Septins

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Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
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Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Sperm Structure and Semen Composition01:22

Sperm Structure and Semen Composition

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During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
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Testes: Histology01:27

Testes: Histology

634
A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
634
Sperm Transport01:15

Sperm Transport

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The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
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Related Experiment Video

Updated: May 26, 2025

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

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SEPT14 complexes maintain sperm morphogenesis and function.

Han-Yu Wang1,2,3, Kuan-Ru Chen4, Bor-Chun Yeh4

  • 1Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|February 21, 2025
PubMed
Summary
This summary is machine-generated.

Septin 14 (SEPT14) is crucial for sperm development and male fertility. Loss of SEPT14 in mice caused infertility with severe sperm defects, highlighting its role in spermatogenesis.

Keywords:
SEPT14capacitationinfertilityseptin filamentsperm annulus

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Genetics

Background:

  • Mutations in the septin (SEPT) gene family are linked to male infertility.
  • Septin 14 (SEPT14) is highly expressed in the testis and reduced in teratozoospermia, suggesting its importance in sperm formation.

Purpose of the Study:

  • To investigate the role of SEPT14 in sperm morphogenesis and function.
  • To elucidate the molecular mechanisms underlying SEPT14's function during spermiogenesis.

Main Methods:

  • Generation and analysis of Septin 14 knockout (Sept14-/-) mice.
  • Microscopic examination of sperm morphology, DNA integrity, mitochondrial organization, and acrosome structure.
  • Immunofluorescence and co-localization studies to identify SEPT14 interacting partners and localization.

Main Results:

  • Sept14-/- male mice exhibited subfertility with abnormal sperm phenotypes, including irregular acrosomes, DNA damage, disorganized mitochondria, and displaced annuli.
  • SEPT14 was localized to the acroplaxome, manchette, neck, and annulus during spermiogenesis.
  • SEPT14 interacts with microtubules and actin during manchette formation and with SEPT9, SEPT7, and SEPT2 in the annulus to maintain its structure.

Conclusions:

  • SEPT14 plays a critical role in regulating sperm morphogenesis and function.
  • SEPT14's interactions with cytoskeletal components and other septins are essential for maintaining sperm structural integrity and fertility.