Downstream signaling induced by several plant Toll/interleukin-1 receptor-containing immune proteins is stable at...

Plant immunity and, in particular, immune responses induced by nucleotide-binding leucine-rich repeat receptors (NLRs) are often dampened above the optimal plant's growth range, but the underlying molecular mechanism remains elusive. N-terminal Toll/interleukin-1 receptor (TIR) domains are self-sufficient to trigger immune signaling. We showed that the conditional activation of two well-characterized TIR-containing NLRs (TNLs) or their corresponding TIR domains alone induce the same signaling route at permissive temperature (ENHANCED DISEASE SUSCEPTIBLITY 1 [EDS1]/helper NLRs that display an RPW8-like N-terminal CC_R domain [RNL] requirement and activation of the salicylic acid sector) in Arabidopsis. Yet, this signaling pathway is maintained under elevated temperatures (30°C) when induced by TIRs only but not full-length TNLs. This work underlines the need to further study how NLRs are impacted by an increase in temperature, which is particularly important to improve the resilience of plant disease resistance in a warming climate.