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Updated: Aug 10, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Definitive identification methods: antibiotic sensitivity and microtube system
Abstract:
The definite identification of species and subspecies of an anaerobe implies certain difficulties, such as the poor and slow fermentation of carbohydrates under anaerobic conditions and the fact of having many keys in common. As a result several tests are required and as the number of tests increases the chances of error and the number of variables also increase. Hence specific methods have been developed in order to try and obtain satisfactory results. The A. refers his experience and judgement on V.P.I. and A.P.I. and on identification based upon antibiotic sensitivity spectrum. When properly used, such techniques enable the species and subspecies of non-sporing anaerobes to be more easily identified.
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