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Related Experiment Videos

A home-made solid-phase sequencer operating at the nanomole level.

I Amiri, J Neimark, A Waltz

    Journal of Biochemical and Biophysical Methods
    |March 1, 1985
    PubMed
    Summary

    A new automated solid-phase sequencer was built for protein sequencing. This cost-effective machine offers comparable sensitivity and efficiency to commercial models for nanomole-level analysis.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Instrumentation

    Background:

    • Automated peptide sequencing is crucial for protein analysis.
    • Existing commercial sequencers can be expensive and complex.
    • Development of accessible, high-performance sequencing technology is needed.

    Purpose of the Study:

    • To describe the construction and operation of a fully automated solid-phase sequencer.
    • To evaluate the performance of the developed sequencer at the nanomole level.
    • To compare its cost, sensitivity, and efficiency against commercial alternatives.

    Main Methods:

    • Peptide coupling to activated glass beads via the alpha-carboxyl end.
    • Sequential reaction with Chang's and Edman's reagents.

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  • Electronic control of all operations using an automated programmer.

    • Main Results:

    • Successful building and functioning of the automated solid-phase sequencer.
    • Demonstrated utility at the nanomole level for protein sequence determination.
    • Favorable comparison in cost, sensitivity, and efficiency with commercial sequencers.

    Conclusions:

    • A fully automated, cost-effective solid-phase sequencer can be constructed from commercially available components.
    • The developed instrument provides a viable alternative to commercial machines for protein sequencing.
    • This technology facilitates nanomole-level protein sequence analysis.