Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interleukin-10 enhances IgG galactosylation and sialylation.

Biomarker research·2026
Same author

Glycosylation profile of <i>Mycobacterium leprae</i>-specific antibodies associated with disseminated infection.

iScience·2026
Same author

Determinants of Immunoglobulin-G N-Glycan Patterns in the Aging General Population.

Allergy·2026
Same author

Antigen-specific IgG glycosylation profiles in hamsters and macaques following COVID-19 vaccination.

iScience·2026
Same author

Mannose receptor contributes to preferential oligomannosidic glycoform clearance in a murine pharmacokinetics model.

mAbs·2026
Same author

Fc Profiling of Polyclonal IgG, IgA and IgM by Light Chain Capturing Coupled with NanoRP-LC-MS.

JACS Au·2026

Related Experiment Video

Updated: May 26, 2025

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

15.5K

Comprehensive Immunoglobulin G, A, and M Glycopeptide Profiling for Large-Scale Biomedical Research.

Bianca D M van Tol1, Anna M Wasynczuk1, Steinar Gijze1

  • 1Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands.

Molecular & Cellular Proteomics : MCP
|February 21, 2025
PubMed
Summary

This study introduces a novel method to analyze antibody glycosylation across IgG, IgA, and IgM isotypes. The technique enables detailed insights into how antibody glycosylation changes in conditions like pregnancy and rheumatoid arthritis.

Keywords:
antibody glycosylationglycopeptidesimmunoglobulinliquid chromatographymass spectrometryrelative quantificationrheumatoid arthritissite-specific glycan analysis

More Related Videos

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

917
Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
11:01

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Published on: January 18, 2020

8.4K

Related Experiment Videos

Last Updated: May 26, 2025

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

15.5K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

917
Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
11:01

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Published on: January 18, 2020

8.4K

Area of Science:

  • Immunology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Antibody glycosylation significantly impacts immune cell functions.
  • IgA and IgM isotypes are increasingly recognized for their roles in disease.
  • Current methods lack the comprehensive analysis of multiple antibody isotypes and their glycosylation patterns.

Purpose of the Study:

  • To develop an integrated method for simultaneous analysis of IgG, IgA, and IgM glycosylation.
  • To enable site-specific quantitation of N- and O-glycosylation across antibody isotypes.
  • To facilitate large-scale clinical studies investigating antibody glycosylation in health and disease.

Main Methods:

  • Simultaneous affinity enrichment of IgG, IgA, and IgM.
  • Glycopeptide-centered liquid chromatography-mass spectrometry (LC-MS).
  • A two-protease approach for peptide generation and MS-based glycoform analysis.

Main Results:

  • Achieved protein-specific (isotype/subclass) and site-specific glycosylation quantitation.
  • Enabled relative quantitation of glycoforms and structural confirmation.
  • Demonstrated application in clinical studies, revealing glycosylation differences in pregnancy and rheumatoid arthritis.

Conclusions:

  • The developed method offers comprehensive, high-resolution analysis of antibody glycosylation.
  • It is precise, robust, and suitable for large clinical cohorts.
  • This approach advances the study of antibody isotype and glycosylation interplay in pathophysiology.