Related Experiment Video

Updated: May 26, 2025

Spontaneous Murine Model of Anaplastic Thyroid Cancer
05:39

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

1.6K

N6-methyladenosine-mediated EIF3H promotes anaplastic thyroid cancer progression and ferroptosis resistance by

Zeyu Zhang1, Di Zhou1, Xiangyuan Qiu1

  • 1Department of Thyroid Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410000, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410000, China.

Free Radical Biology & Medicine
|February 21, 2025
PubMed

Insights

EIF3H deubiquitylates and stabilizes beta-catenin, promoting anaplastic thyroid cancer (ATC) progression and ferroptosis resistance. Targeting the EIF3H/beta-catenin axis offers a potential therapeutic strategy for ATC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Anaplastic thyroid cancer (ATC) has a poor prognosis and limited treatment options.
  • The Wnt/beta-catenin pathway is crucial in ATC, but regulatory mechanisms are not fully understood.
  • The role of the JAMM family of deubiquitinating enzymes in ATC is unknown.

Purpose of the Study:

  • To identify a novel deubiquitinating enzyme that regulates beta-catenin in ATC.
  • To elucidate the mechanism by which this enzyme affects beta-catenin stability and Wnt/beta-catenin signaling.
  • To investigate the therapeutic potential of targeting this enzyme in ATC.

Main Methods:

  • Correlation analysis between EIF3H and beta-catenin expression in ATC.
  • EIF3H knockdown experiments to assess effects on Wnt/beta-catenin signaling.
  • Biochemical assays to confirm interaction, deubiquitylation, and stabilization of beta-catenin by EIF3H.
  • Assessment of EIF3H's impact on ATC cell proliferation, invasion, and ferroptosis resistance.
  • Investigation of EIF3H's association with m6A modification and IGF2BP2.

Main Results:

  • EIF3H expression positively correlates with beta-catenin levels in ATC.
  • EIF3H knockdown inhibits the Wnt/beta-catenin pathway, reducing ATC cell proliferation and invasion.
  • EIF3H directly deubiquitylates and stabilizes beta-catenin by removing K48-linked ubiquitin chains.
  • EIF3H knockdown enhances ferroptosis sensitivity in ATC cells.
  • EIF3H dysregulation is linked to m6A modification and the m6A reader IGF2BP2.

Conclusions:

  • The EIF3H/beta-catenin axis promotes ATC progression and ferroptosis resistance via Wnt/beta-catenin signaling activation.
  • EIF3H acts as a deubiquitinating enzyme targeting beta-catenin.
  • The EIF3H/beta-catenin axis presents a potential diagnostic marker and therapeutic target for ATC.
Keywords:
Anaplastic thyroid cancerFerroptosisUbiquitylationm6A modification

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice00:07

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.2K
Induction and Analysis of Epithelial to Mesenchymal Transition10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

35.7K

Related Concept Videos

Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
2.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.7K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.6K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K