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AjMALT1 promotes Vibrio splendidus-induced inflammation through the NF-κB pathway in Apostichopus japonicus

Haiping Liu1, Jiping Wang1, Weiwei Zhang1

  • 1Key Laboratory of Aquacultural Biotechnology (Ningbo University), Ministry of Education, Ningbo, 315800, PR China.

Developmental and Comparative Immunology
|February 21, 2025
PubMed

Insights

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) in sea cucumbers exacerbates inflammation. Silencing AjMALT1 reduced inflammatory responses and cytokine expression, indicating its role in the immune pathway.

Area of Science:

  • Marine biology
  • Immunology
  • Molecular biology

Background:

  • Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) is a key signaling molecule in inflammatory responses.
  • Understanding the role of MALT1 in invertebrate immunity is crucial for marine animal health.

Purpose of the Study:

  • To investigate the immune function of AjMALT1 in the sea cucumber Apostichopus japonicus.
  • To elucidate the molecular mechanisms underlying AjMALT1-mediated inflammation in A. japonicus.

Main Methods:

  • Cloning of the full-length AjMALT1 gene using transcriptome data and RACE technology.
  • Quantitative gene expression analysis of AjMALT1, AjIL17, and AjNLRP3.
  • RNA interference (siRNA) to silence AjMALT1 expression.
  • Histological examination of intestinal tissues.
  • Analysis of AjTRAF6 and AjRel expression and nuclear translocation.

Main Results:

  • AjMALT1 is expressed in all tissues of A. japonicus, with higher levels in coelomocytes and the intestine.
  • AjMALT1 expression is upregulated during Vibrio splendidus infection and correlates with pro-inflammatory cytokines AjIL17 and AjNLRP3.
  • Silencing AjMALT1 reduces AjIL17 and AjNLRP3 expression, alleviates intestinal inflammation, and inhibits the AjTRAF6-dependent NF-κB pathway.

Conclusions:

  • AjMALT1 plays a significant role in exacerbating intestinal and coelomic inflammation in A. japonicus.
  • The AjTRAF6-dependent NF-κB pathway is implicated in AjMALT1-mediated inflammatory responses.
  • AjMALT1 is a potential target for managing inflammatory diseases in aquaculture.
Keywords:
Apostichopus japonicusImmune responseInflammationMALT1Vibrio splendidus

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