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Updated: Aug 7, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Halofenate and clofibrate inhibition of pyruvate dehydrogenase from Fusarium culmorum
Abstract:
Pyruvate dehydrogenase (E1, E.C. 1.2.4.1) was obtained from Fusarium culmorum by ammonium sulfate precipitation. An eight-fold purification was obtained with a specific activity of 13 K units/mg protein. Both halofenate and clofibrate inhibited the enzyme complex non-competitively. The inhibitory effect of halofenate was greater than that of clofibrate being 42% higher at 20 mM concentration compared to the inhibition by clofibrate at 40 mM concentration. Both compounds disorganized the normal cytoplasmic lipids including the emptying of cells in the mycelium suggesting membrane disruption.
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