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Updated: Aug 15, 2026
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Ultrasensitive detection of Vibrio parahaemolyticus based on boric acid-functionalized Eu (III)-based metal-organic
Jingyi Xiao1, Yi Ren1, Menglong Liu1
1College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province. Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities. Jinzhou, Liaoning, 121013, China.
Abstract:
This study intends to create a ratiometric fluorescence probe utilizing aptamers for the detection of Vibrio parahaemolyticus (V. parahaemolyticus) in aquatic products. In this design, aptamer-functionalized magnetic nanoparticles specifically capture V. parahaemolyticus, while boric acid on Eu (III)-Based Metal-Organic Framework (Eu-MOF) interacts with glycolipids present on bacterial cells, thereby achieving dual recognition of V. parahaemolyticus. This fluorescent probe quantitatively detects V. parahaemolyticus by measuring the intensity of ratio fluorescence. The sensor demonstrates a detection range from 77 to 7.7 × 107 CFU/mL, possessing a detection threshold down to 1 CFU/mL. Moreover, the developed method based on Eu-MOF had been successfully applied to real samples. To achieve rapid on-site detection of V. parahaemolyticus, the study designed a portable smartphone sensor that confirms its capability for rapidly detecting pathogens and contributes significantly to establishing a system for regulating safety in detecting food and environment.
