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Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
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Immunogold technique applied to simultaneous identification of T6 and HLA-DR antigens on Langerhans cells by electron

C Dezutter-Dambuyant, D Schmitt, M Faure

    The Journal of Investigative Dermatology
    |June 1, 1985
    PubMed

    A double-labeling immunogold technique in electron microscopy and specific monoclonal antibodies to surface antigens of Langerhans cells (OKT6 and BL2) were applied to assess directly the coexpression of two cell surface antigens (T6 and HLA-DR antigens) in a heterogeneous epidermal cell suspension. Electron microscopic examination of double-labeled cells revealed that all Birbeck granule-containing Langerhans cells bound OKT6 and BL2. The preparation of markers with colloidal gold particles and the procedure for double labeling are described. Several problems related to the steric hindrance and current artifacts are illustrated by micrographs and also discussed.

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    Immunogold Electron Microscopy01:20

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    Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.