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Differential Expression of MRPS18 Family Genes in Brain Tumor Samples
L Kovalevska1, S Kalman1, A Sushnova1
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine.
Experimental Oncology
|February 22, 2025
Summary
MRPS18 family genes show distinct expression patterns in brain tumors and blood. MRPS18-1 is highest in glioblastoma, while MRPS18-2 shows high protein levels across most brain tumors, aiding in molecular profiling.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Brain tumors represent a significant portion of central nervous system neoplasms, with a notable 5-year survival rate.
- Identifying novel biomarkers is crucial for refining the molecular classification and improving the prognostic accuracy of brain tumors.
Purpose of the Study:
- To investigate the differential gene expression of the MRPS18 family in brain tumor tissues and patient peripheral blood.
- To correlate gene expression patterns with brain tumor types and grades.
Main Methods:
- Quantitative polymerase chain reaction (qPCR) was used to analyze mRNA expression of MRPS18 family genes.
- Immunohistochemical (IHC) studies were performed to assess MRPS18 protein expression in tumor tissues.
- Samples were obtained from 27 patients diagnosed with various brain tumors.
Main Results:
- MRPS18-1, MRPS18-2, and MRPS18-3 genes exhibited high mRNA expression in both tumor tissues and peripheral blood.
- Differential expression patterns were observed across various tumor types and grades, with MRPS18-1 notably high in glioblastoma (G4 tumors).
- MRPS18-2 showed high protein expression in most brain tumors, irrespective of grade or type.
Conclusions:
- MRPS18 family genes display consistent mRNA expression patterns in brain tumor tissue and peripheral blood.
- MRPS18-1 mRNA levels were highest in glioblastoma, while MRPS18-2 protein levels were elevated across most brain tumors, suggesting potential as biomarkers.
