Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

1.2K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
1.2K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

398
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
398
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

1.3K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
1.3K
Data Validation01:15

Data Validation

141
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
141
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

1.4K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
1.4K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

324
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
324

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In-situ</i> gel drug delivery system as a viable approach to periodontal therapy: A comprehensive review.

Journal of advanced periodontology & implant dentistry·2025
Same author

Inhalation beyond respiratory: the surprising applications of dry powder inhalers.

Monaldi archives for chest disease = Archivio Monaldi per le malattie del torace·2025
Same author

Chiral Inversion of Pharmaceutical Drugs - Mini Review.

Combinatorial chemistry & high throughput screening·2023
Same author

D-Optimal mixture design optimization of an HPLC method for simultaneous determination of commonly used antihistaminic parent molecules and their active metabolites in human serum and urine.

Biomedical chromatography : BMC·2017

Related Experiment Video

Updated: May 26, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.4K

Navigating the AQbD Landscape: Enhancing Quality Management in Liquid Chromatography Method Development.

Selvakumar Kanthiah1, J Joysa Ruby2, Hiriyanna Sgb3

  • 1Head of the Department of Quality Assurance, Acharya & BM Reddy College of Pharmacy, Bengaluru, Karnataka, India.

Biomedical Chromatography : BMC
|February 22, 2025
PubMed
Summary

Analytical Quality by Design (AQbD) offers a scientific approach to liquid chromatography (LC) method development, ensuring quality and regulatory compliance. This review details AQbD principles, components, and applications for robust HPLC method lifecycles.

Keywords:
AQbDlife cycleliquid chromatographypharmaceutical drugsquality management

More Related Videos

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.6K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

538

Related Experiment Videos

Last Updated: May 26, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.4K
Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.6K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

538

Area of Science:

  • Pharmaceutical Analysis
  • Analytical Chemistry

Background:

  • Analytical Quality by Design (AQbD) is a systematic approach to LC method development.
  • It emphasizes understanding method variables and their impact on quality.

Purpose of the Study:

  • To review AQbD principles and methodology in HPLC method lifecycle.
  • To discuss AQbD components, regulatory perspectives, and outcomes in pharmaceutical analysis.

Main Methods:

  • Defining Analytical Target Profile (ATP), Critical Method Parameters (CMPs), and Critical Quality Attributes (CQAs).
  • Utilizing Design of Experiments (DoE) for risk assessment and optimization.
  • Establishing an analytical design space and control strategy.

Main Results:

  • AQbD facilitates a comprehensive understanding of HPLC method development and lifecycle management.
  • Key components include ATP, CQA, Method Operable Design Region (MODR), and control strategies.
  • Continuous method monitoring is crucial for sustained quality.

Conclusions:

  • AQbD is essential for pharmaceutical quality assurance and regulatory compliance in LC method development.
  • Understanding AQbD principles, applications, and regulatory aspects is vital.
  • AQbD implementation leads to robust and continuously improving analytical methods.