Related Experiment Video
Updated: May 26, 2025
Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy
Published on: May 27, 2020
Test-retest reliability of coupling between cerebrospinal fluid flow and global brain activity after normal sleep and
Weiwei Zhao1, Joy Rao2, Ruosi Wang1
1Center for Magnetic Resonance Imaging Research & Key Laboratory of Brain-Machine Intelligence for Information Behavior (Ministry of Education and Shanghai), School of Business and Management, Shanghai International Studies University, Shanghai, PR China.
Abstract:
The glymphatic system (GS) plays a key role in maintaining brain homeostasis by clearing metabolic waste during sleep, with the coupling between global blood-oxygen-level-dependent (gBOLD) and cerebrospinal fluid (CSF) signals serving as a potential marker for glymphatic clearance function. However, the test-retest reliability and spatial heterogeneity of gBOLD-CSF coupling after different sleep conditions remain unclear. In this study, we assessed the test-retest reliability of gBOLD-CSF coupling following either normal sleep or total sleep deprivation (TSD) in 64 healthy adults under controlled laboratory conditions. The reliability was high after normal sleep (ICC = 0.763) but decreased following TSD (ICC = 0.581). Moreover, spatial heterogeneity was evident in participants with normal sleep, with lower-order networks (visual, somatomotor, and attention) showing higher ICC values compared to higher-order networks (default-mode, limbic, and frontoparietal). This spatial variation was less distinct in the TSD group. These results demonstrate the robustness of the gBOLD-CSF coupling method and emphasize the significance of considering sleep history in glymphatic function research.
