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Production of caproate from lactate by chain elongation under electro-fermentation: Dual role of exogenous ethanol
Jihua Zhao1, Hongzhi Ma2, Ming Gao1
1Department of Environmental Science and Engineering, University of Science and Technology Beijing, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China.
Abstract:
Ethanol and lactate as co-electron donors (co-EDs) on chain elongation (CE) have been studied, however, the effects of ethanol on lactate-driven CE remain unknown. This study evaluated the caproate production performance from lactate under different ethanol additions (7.67-23.01 g/L), and determined the optimal ethanol concentration. The results showed that the highest caproate yield of 4.51 g/L was obtained at 15.34 g/L ethanol concentration. The synergistic effects of co-EDs on caproate production were enhanced due to more stable pH and higher reducing power under appropriate ethanol stimulation. Functional bacteria related to caproate production, including Sporanaerobacter and Clostridium_senseu_stricto_12, were specifically enriched under appropriate ethanol stimulation. The ethanol toxicity caused by excessive concentration (more than 23.01 g/L) inhibited the growth of key CE bacteria and reduced the production of caproate. This study revealed the dual effects of ethanol in the co-EDs fermentation system for caproate production and determined the threshold of ethanol addition.
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