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HOXA10-AS Enhances Gastric Cancer Cell Proliferation, Migration, and Invasion via the p38 MAPK/STAT3 Signaling Pathway

  • 0Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, China.
Journal of Biochemical and Molecular Toxicology +

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February 23, 2025

|

February 23, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Long noncoding RNA HOXA10-AS promotes gastric cancer (GC) progression by upregulating HOXA10 expression, which activates the p38 MAPK/STAT3 pathway. This study reveals HOXA10-AS as a potential therapeutic target for gastric cancer.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genetics

Background

  • Gastric cancer (GC) is a significant global health issue, with over 1 million new cases annually.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their role in GC progression.

Purpose Of The Study

  • To investigate the role and mechanism of lncRNA HOXA10 antisense RNA (HOXA10-AS) in modulating the malignant properties of GC cells.
  • To elucidate the regulatory pathway involving HOXA10-AS, HOXA10, and miR-29a/b/c-3p in GC.

Main Methods

  • Quantitative reverse transcription PCR (RT-qPCR) for gene expression analysis.
  • Cellular assays (colony formation, Transwell) to assess cell proliferation, invasion, and migration.
  • Molecular techniques including RNA immunoprecipitation, RNA pulldown, and luciferase assays to determine gene interactions.
  • Western blot analysis for epithelial-mesenchymal transition (EMT) markers.

Main Results

  • HOXA10-AS was highly expressed in GC cells and its silencing reduced proliferation, invasion, migration, and EMT.
  • HOXA10-AS positively regulated HOXA10 expression via interaction with miR-29a/b/c-3p.
  • Overexpression of HOXA10 reversed the inhibitory effects of HOXA10-AS knockdown.
  • HOXA10-AS activated the p38 MAPK/STAT3 signaling pathway by upregulating HOXA10.

Conclusions

  • HOXA10-AS promotes GC cell growth, migration, invasion, and EMT by upregulating HOXA10 expression.
  • The HOXA10-AS/miR-29a/b/c-3p/HOXA10 axis activates the p38 MAPK/STAT3 signaling pathway, driving GC progression.
  • HOXA10-AS represents a potential therapeutic target for gastric cancer treatment.

Keywords:

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