Related Experiment Video
Updated: May 11, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
Development of coumarin and procaine linked hybrid molecules as a novel class of SHP1 fluorescent activators
Xu-Yang Mu1, Li-Xin Gao2, Zhen-Xuan Zhang3
1School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China; School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
Abstract:
The development of small fluorescent organic molecules used in bioimaging experiment has boomed the progress of molecular and cellular biology, providing new and efficient tools to elucidate a myriad of cellular and multicellular processes. In this work, a class of fluorescent activators against SHP1 was designed and synthesized for the first time. The representative compound 3n showed activating effect against SHP1 with EC50 of 17.66 ± 1.48 μM and a fluorescence quantum yield of 0.521 in DMSO. Meanwhile, 3n showed good selectivity for SHP1, inhibited the proliferation of SU-DHL-2 cells and OCI-Ly10 cells with IC50 of 8.66 ± 1.26 μM and 9.16 ± 0.53 μM and exhibited potential for cellular imaging on human breast cancer cells.

