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Profiling Proteins Involved in Peroxynitrite Homeostasis Using ROS/RNS Conditional Proteomics
Hao Zhu1, Hiroaki Uno1, Kyoichi Matsuba1
1Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Journal of the American Chemical Society
|February 24, 2025
Summary
Researchers developed novel Peroxynitrite-responsive protein Labeling reagents (Porp-L) to identify proteins interacting with peroxynitrite (ONOO⁻). This new tool revealed the endoplasmic reticulum as a key site for ONOO⁻ activity and identified Ero1a
Area of Science:
- Biochemistry
- Cell Biology
- Proteomics
Background:
- Peroxynitrite (ONOO⁻) is crucial in oxidative stress and redox signaling.
- Existing methods for ONOO⁻ detection lack specificity and sensitivity.
- High-throughput profiling of ONOO⁻-associated proteins is challenging.
Purpose of the Study:
- To develop a novel conditional proteomics approach for investigating ONOO⁻ homeostasis.
- To identify protein neighbors of ONOO⁻ within cells.
- To discover new roles for proteins in ONOO⁻ biology.
Main Methods:
- Development of Peroxynitrite-responsive protein Labeling reagents (Porp-L).
- Utilized 2,6-dichlorophenol as an ONOO⁻-activatable moiety for protein labeling.
- Applied Porp-L based conditional proteomics in immune-stimulated macrophages.
Main Results:
- Identified proteins involved in ONOO⁻ generation and modification.
- Revealed the endoplasmic reticulum (ER) as a significant ONOO⁻ hot spot.
- Discovered a novel role for Ero1a in ONOO⁻ formation.
Conclusions:
- Porp-L reagents offer a promising tool for studying ONOO⁻ biology.
- Conditional proteomics with Porp-L advances understanding of ONOO⁻ homeostasis.
- The study highlights the ER and Ero1a in ONOO⁻-related cellular processes.
