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Updated: May 26, 2025
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Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
Published on: April 1, 2022
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Quantifying the relationship between cell proliferation and morphology during development of the face
Lucas D Lo Vercio1,2,3, Rebecca M Green4, Andreas Dauter1,2,3
1Department of Cell Biology & Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.
Development (Cambridge, England)
|February 24, 2025
Summary
Understanding how cell behaviors like proliferation drive embryonic development is key. This study developed a 3D imaging pipeline to link cell proliferation and tissue shape changes in developing mouse faces.
Area of Science:
- Developmental Biology
- Biophysics
- Computational Biology
Background:
- Morphogenesis involves complex cellular processes (proliferation, migration, apoptosis) and tissue interactions.
- Understanding the link between cellular dynamics and 3D tissue shape changes in development remains a challenge.
- Existing 3D imaging methods struggle with high-throughput quantification of cellular and tissue dynamics.
Purpose of the Study:
- To develop and validate an imaging and analysis pipeline for 3D quantification of cellular dynamics and morphology in developing embryos.
- To investigate the relationship between the 3D distribution of cell proliferation and morphogenesis during mouse facial development.
Main Methods:
- Light-sheet microscopy for 3D imaging of individual embryos.
- Machine learning-based tools for automated segmentation of cells and tissues.
- Geometric morphometrics for quantifying external embryonic morphology.
- Integrated pipeline for 3D quantification of cellular dynamics and morphology.
Main Results:
- Demonstrated a pipeline enabling simultaneous 3D quantification of cellular dynamics and morphology.
- Showed a tight correlation between changes in cell proliferation and morphological changes during mouse facial development.
- Validated the pipeline's potential for mechanistic investigations.
Conclusions:
- The developed pipeline facilitates robust 3D quantification of cellular and tissue dynamics.
- Cell proliferation is a key factor tightly associated with facial morphogenesis.
- This approach offers a powerful framework for studying the interplay between cellular behaviors and developmental processes.
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