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Exploring Molecular Alterations in Breast Cancer Among Indian Women Using Label-Free Quantitative Serum Proteomics

  • 0Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
Biochemistry Research International +

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February 24, 2025

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February 24, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study identified novel protein biomarkers in Indian women with breast cancer. These potential diagnostic markers, including SBSN and PFN1, could improve early detection and targeted therapies for breast cancer.

Area Of Science

  • Biochemistry
  • Oncology
  • Proteomics

Background

  • Breast cancer presents significant challenges in India due to high incidence and mortality rates.
  • Early detection and effective screening are critical to reduce breast cancer-related deaths.
  • Quantitative proteomic analysis offers a powerful approach for identifying cancer biomarkers and guiding therapeutic strategies.

Purpose Of The Study

  • To identify and characterize protein markers associated with breast cancer in Indian women.
  • To discover potential diagnostic tools and therapeutic targets for breast cancer.
  • To analyze protein expression patterns linked to disease aggressiveness and metastatic potential.

Main Methods

  • Utilized a label-free quantitative proteomic method on serum samples.
  • Employed statistical analysis to identify differentially expressed proteins (DEPs) between breast cancer patients and controls.
  • Validated the expression of specific proteins, such as SBSN, using western blotting.

Main Results

  • Identified several DEPs in breast cancer patients compared to controls, including upregulated proteins (SBSN, ANG, PCOLCE, WFDC3) and downregulated proteins (PFN1, FLNA, DSG2).
  • Demonstrated that DEPs can effectively distinguish between breast cancer and control samples.
  • Observed progressive changes in SBSN (upregulation) and PFN1 (downregulation) expression across different stages of breast cancer.

Conclusions

  • Recognized SBSN and PFN1 as promising prospective biomarkers for breast cancer in Indian women.
  • Highlighted the potential of identified DEPs as diagnostic tools and therapeutic targets.
  • Emphasized the need for further functional studies to elucidate the precise roles of these proteins in breast cancer development.