Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translating Blue Light Stimulation From Batch to Perfusion: Process and Intracellular Metabolic Analysis.

Biotechnology and bioengineering·2026
Same author

A Method for Optical Quantification of Local Oxygen Limitations in Multiphase Bioreactors.

Biotechnology and bioengineering·2026
Same author

Ensuring Good Transferability from Pilot- to Large-Scale Optimized Biotech Bubble Column Designs.

Bioengineering (Basel, Switzerland)·2026
Same author

Multivariate phase-dependent optimization of bioprocesses boosts performance and quality-Why timing (of exposure) matters.

Biotechnology progress·2026
Same authorSame journal

Less Genome, More Gain: Genome Reduction Enhances Transaminase-Producing <i>E. coli</i> in a Scale-Down Bioreactor.

Engineering in life sciences·2026
Same author

Overriding Bioprocess Perturbations With a Cell-Machine Interface for Reliable Microbial Stress-Response Control.

Microbial biotechnology·2026
Same journal

Filament Extrusion-Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro.

Engineering in life sciences·2026
Same journal

Design and Characterization of a 3D-Printable Membrane Aeration Module for Small-Scale Bioprocess Prototyping.

Engineering in life sciences·2026
Same journal

Molecular Pharming: Advances, Applications, and Future Prospects in Biotechnology and Medicine.

Engineering in life sciences·2026
Same journal

Topic: Continuous Enzymatic Peracids Synthesis in Pickering Emulsions: Influence of Nanoparticles Modification, Aqueous Phase Composition and Operational Parameters.

Engineering in life sciences·2026
Same journal

Optimized Bioconversion of Naringenin to Hesperetin in <i>Escherichia coli</i> With Halide Methyltransferase-Mediated <i>S</i>-Adenosylmethionine Regeneration.

Engineering in life sciences·2026
See all related articles

Related Experiment Video

Updated: May 26, 2025

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
10:45

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

12.8K

Mini Bubble Columns for Miniaturizing Scale-Down.

Moritz Wild1, Ralf Takors1

  • 1Institute of Biochemical Engineering University of Stuttgart Stuttgart Germany.

Engineering in Life Sciences
|February 24, 2025
PubMed
Summary
This summary is machine-generated.

A new miniaturized bubble column reactor effectively simulates industrial fermentation conditions, enabling faster and more efficient strain selection for biotechnological processes. This scale-down simulator accurately predicts host performance under stress.

Keywords:
chemostat fermentationmini bubble column reactornutrient starvationscale‐downscale‐up

More Related Videos

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
13:59

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Published on: November 13, 2014

13.7K

Related Experiment Videos

Last Updated: May 26, 2025

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
10:45

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

12.8K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

11.7K
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
13:59

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

Published on: November 13, 2014

13.7K

Area of Science:

  • Biotechnology and Bioprocess Engineering
  • Microbial Physiology
  • Synthetic Biology

Background:

  • Scaling up biotechnological processes from lab to industrial scale is critical for innovation.
  • Scale-down simulators are essential for evaluating host adaptability in large fermenters.
  • Conventional scale-down systems are often complex, time-consuming, and resource-intensive.

Purpose of the Study:

  • To introduce a miniaturized bubble column reactor as an efficient scale-down simulator.
  • To assess the system's ability to mimic industrial fermentation conditions and cellular responses.
  • To validate the system's performance against conventional stirred tank reactor (STR) and plug-flow reactor (PFR) setups.

Main Methods:

  • Development of a 60 mL working volume bubble column reactor for parallel, carbon-limited chemostat fermentations.
  • Subjecting *Escherichia coli* to cyclic carbon starvation and reintroduction.
  • Characterization of cellular responses using guanosine pentaphosphate (ppGpp) accumulation, energy charge measurements, and transcriptomic analysis.

Main Results:

  • The system successfully simulated carbon starvation and reintroduction, inducing ppGpp accumulation and energy charge fluctuations in *E. coli*.
  • Transcriptomic analysis revealed a two-phase gene expression response, mediated by CRP-cAMP, ppGpp, and stress sigma factors.
  • Data generated by the miniaturized system closely matched results from conventional STR/PFR setups.

Conclusions:

  • The miniaturized bubble column reactor is a valid and efficient scale-down simulator for biotechnological processes.
  • This system streamlines strain development by providing accurate predictions of host performance.
  • It offers a resource-efficient alternative for evaluating microbial responses to dynamic industrial fermentation conditions.