Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.1K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.1K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

5.0K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
5.0K
Oogenesis01:22

Oogenesis

949
Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
949
Stem Cell Niche01:26

Stem Cell Niche

5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision Immunoregulation in transplantation: The rise of engineered Treg therapies.

Molecular biology reports·2026
Same author

Immune-antioxidant potential, splenic cytokines regulation, and tissue architecture restoration by grape seed oil nanoemulsion in Nile tilapia subjected to acrylamide toxicity.

Fish physiology and biochemistry·2026
Same author

A review of recent advances in exosome-mediated drug delivery for regenerative therapy and immunomodulation.

Biomedical engineering online·2026
Same author

Bioengineering Pancreatic Organoids and iPSC-Derived β-Cells for Diabetes: Materials, Devices, and Translational Challenges.

Bioengineering (Basel, Switzerland)·2026
Same author

Public Health Impact and Cost-Effectiveness of 2-Dose vs 1-Dose Human Papillomavirus Vaccination Regimen in Saudi Arabia.

Journal of health economics and outcomes research·2026
Same author

Pathogenic homozygous PLCZ1 mutation reduces sperm protein levels with a simulated reduction of PIP2 binding: a case report.

Reproduction & fertility·2026

Related Experiment Video

Updated: May 26, 2025

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
08:39

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Published on: January 3, 2015

9.5K

The Mammalian Oocyte: A Central Hub for Cellular Reprogramming and Stemness.

Islam M Saadeldin1,2, Seif Ehab3, Mashan Essa F Alshammari4

  • 1Comparative Medicine Department, King Faisal Specialist Hospital and Research Centre, Riyadh, 11211, Saudi Arabia.

Stem Cells and Cloning : Advances and Applications
|February 24, 2025
PubMed
Summary

Mammalian oocytes drive cellular reprogramming and stemness, resetting cell identity for pluripotency. Understanding oocyte factors advances regenerative medicine, cloning, and stem cell therapies.

Keywords:
epigeneticsgenome activationoocytesreprogramming

More Related Videos

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

13.7K
Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
09:47

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

9.2K

Related Experiment Videos

Last Updated: May 26, 2025

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
08:39

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Published on: January 3, 2015

9.5K
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
11:37

In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells

Published on: February 18, 2015

13.7K
Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
09:47

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

9.2K

Area of Science:

  • Developmental Biology
  • Reproductive Biology
  • Cellular Biology

Background:

  • Mammalian oocytes are crucial for reproduction, providing half the nuclear genome and all mitochondrial DNA.
  • They initiate cellular reprogramming, resetting somatic cell identity to pluripotency, vital for development and health.
  • Oocyte reprogramming is key to understanding cellular differentiation, assisted reproduction, cloning, and stem cell research.

Purpose of the Study:

  • To explore the pivotal role of mammalian oocytes in cellular reprogramming and stemness.
  • To elucidate the mechanisms by which oocytes facilitate nuclear remodeling, epigenetic modifications, and metabolic reprogramming.
  • To highlight the significance of oocyte components and their contribution to reprogramming efficiency and cellular homeostasis.

Main Methods:

  • Review of existing literature on oocyte biology and reprogramming mechanisms.
  • Analysis of the contribution of oocyte-derived components (e.g., mitochondria, nanovesicles, ELVAS) to reprogramming.
  • Examination of the maternal-zygotic transition and its role in establishing totipotency.

Main Results:

  • Oocytes possess inherent reprogramming factors essential for early embryogenesis.
  • Specific oocyte structures like mitochondria and novel components (ooplasmic nanovesicles, ELVAS) are critical for homeostasis and reprogramming.
  • The maternal-zygotic transition shifts developmental control, establishing totipotency.

Conclusions:

  • Unraveling oocyte reprogramming mechanisms deepens our understanding of cloning, cell differentiation, and stem cell therapy.
  • Oocytes are central to developmental biology and regenerative medicine due to their unique reprogramming capabilities.
  • Further research into oocyte components promises advancements in assisted reproductive technologies and regenerative medicine.