Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

294
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
294
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

282
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
282
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

459
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
459

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coronamicroparticle Arrays with Stable Superamphiphobicity.

Small methods·2026
Same author

Interface charge engineering of ternary RuCoMo oxide nanofibers toward high-current-density water electrolysis.

Chemical science·2026
Same author

Beyond Color: Hybrid Vibrational-Electronic Broadband Coherent Anti-Stokes Raman Scattering for Molecularly Informed Digital Pathology.

Analytical chemistry·2026
Same author

Design of a triplex fluorescence aptasensor for a culture-free diagnosis of peritoneal dialysis-related peritonitis.

Scientific reports·2026
Same author

AirNet: A Deep Learning-Driven Auto Baseline Correction Algorithm Balancing Global Smoothness and Local Fidelity.

Analytical chemistry·2026
Same author

Label-Free SERS Detection of Molecular Stress Response in the Golgi Apparatus Induced by Pulsed Electrical Stimulation during Neuronal Cell Differentiation.

Analytical chemistry·2026
Same journal

In Situ N-Doped Carbon Coating on NaTi<sub>2</sub>(PO<sub>4</sub>)<sub>3</sub> for Aqueous Hybrid-Ion Batteries Mitigating Hydrogen Evolution Reactions and Material Dissolution.

ACS applied materials & interfaces·2026
Same journal

Micro-Cluster Engineering Enables Hybrid Channel-Network Cracks for Highly Sensitive, Linear, and Robust Strain Sensors.

ACS applied materials & interfaces·2026
Same journal

3D Bismuth Anode with Synergistic Structural and Interfacial Optimization for High-Performance Sodium-Ion Capacitors.

ACS applied materials & interfaces·2026
Same journal

Facile Synthesis of Stable Yb<sup>3+</sup>-Doped Perovskite Nanocrystals in Mesoporous Silica for Near-Infrared Emission.

ACS applied materials & interfaces·2026
Same journal

The Flexible Sound Source.

ACS applied materials & interfaces·2026
Same journal

Design and Fabrication of PS-PMMA-Based Plastic Scintillators with Bis(pinacolato)diboron Loading for n/γ Pulse-Shape Discrimination.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: May 26, 2025

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K

Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges.

Linley Li Lin1, Ramon Alvarez-Puebla2,3, Luis M Liz-Marzán4,5,6,7

  • 1Sixth People's Hospital, School of Medicine & School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. China.

ACS Applied Materials & Interfaces
|February 24, 2025
PubMed
Summary
This summary is machine-generated.

Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for biomedical analysis. This review covers SERS advancements in substrates, nanotags, and applications for diagnostics and deep tissue imaging.

Keywords:
Raman scatteringSERSomemetabolic detectionnanomedicineplasmonicstransmission Raman spectroscopy

More Related Videos

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.3K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

Related Experiment Videos

Last Updated: May 26, 2025

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.3K
Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

1.5K

Area of Science:

  • Biomedical Spectroscopy
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Surface-enhanced Raman spectroscopy (SERS) has become a critical biomedical tool due to its high sensitivity and molecular specificity.
  • 2024 marks the 50th anniversary of SERS discovery, highlighting its significant evolution.

Purpose of the Study:

  • To review recent advancements and challenges in SERS for biomedical applications.
  • To discuss the potential of SERS in diagnostics, single-cell analysis, and in vivo imaging.

Main Methods:

  • Review of key developments in SERS substrates (colloidal, solid, hydrogel architectures).
  • Exploration of innovations in SERS nanotags (interior gaps, NIR-II responsive, biomimetic coatings).
  • Discussion of emerging technologies (optical tweezers, plasmonic nanopores, wearable sensors) and spectral analysis (deep learning).

Main Results:

  • Highlighting progress in SERS substrates with improved surface chemistry and hotspot design.
  • Introducing novel SERS nanotags with enhanced functionalities for biological detection.
  • Demonstrating expanded SERS capabilities for single-cell/molecule analysis and improved data quantification.

Conclusions:

  • SERS shows significant potential in nucleic acid detection, protein/metabolite analysis, and single-cell monitoring.
  • Emerging applications include liquid biopsy, metabolic phenotyping, and extracellular vesicle diagnostics.
  • Clinical translation requires addressing challenges in in vivo sensing and commercialization.