Ginsenoside CK Promotes Cell Apoptosis via Regulating P53 Signal Pathway in Non-Small Cell Lung Cancer

Area of Science:

• Oncology
• Pharmacology
• Molecular Biology

Background:

• Non-small cell lung cancer (NSCLC) presents significant global health challenges due to high morbidity and mortality.
• Ginsenosides are natural compounds with demonstrated antitumor properties, including the inhibition of tumor cell proliferation and induction of apoptosis.

Purpose of the Study:

• To investigate the effects of ginsenoside CK on the proliferation and apoptosis of NSCLC cell lines (95D and NCI-H460).
• To elucidate the underlying molecular mechanism of ginsenoside CK's anti-NSCLC activity.

Main Methods:

• Cell proliferation was assessed using CCK8 and colony formation assays.
• Apoptosis was analyzed via flow cytometry and fluorescence staining.
• The involvement of the mitochondrial apoptosis pathway was examined using Western Blot analysis.

Main Results:

• Ginsenoside CK significantly inhibited the proliferation and growth of both 95D and NCI-H460 NSCLC cells.
• The compound was found to induce apoptosis in NSCLC cells through the mitochondrial pathway.
• Western Blot analysis confirmed the activation of key proteins in the mitochondrial apoptosis cascade.

Conclusions:

• Ginsenoside CK exhibits potent anti-proliferative and pro-apoptotic effects on non-small cell lung cancer cells.
• The mechanism of action is mediated via the mitochondrial apoptosis pathway.
• Ginsenoside CK represents a promising therapeutic candidate for NSCLC and potentially other major tumor diseases.