miR-379-5p Inhibited the Proliferation of Acute Myeloid Leukemia Cells Through Negative Regulation of YBX1

Acute myeloid leukemia (AML) highly lethal hematological malignancy that is difficult to treat. This study aimed to clarify the molecular mechanisms of miR-379-5p in AML progression.

Quantitative real-time polymerase chain reaction was utilized to evaluate miR-379-5p expression levels in AML patients and a control group. A receiver operating characteristic curve was created to assess the clinical predictive value of miR-379-5p in AML, while cell experiments used the CCK-8 assay, flow cytometry, and transwell chambers. Potential target genes of miR-379-5p were predicted by employing online bioinformatics tools, followed by validation using a dual luciferase reporter assay.

miR-379-5p expression was significantly decreased in AML patients and had clinical predictive value for the disease. In AML cell lines, miR-379-5p was downregulated; conversely, the upregulation of miR-379-5p inhibited proliferation, migration, and invasion while promoting apoptosis. Notably, YBX1 was a potential target gene of miR-379-5p and its upregulation reduced the effects of miR-379-5p on AML cell behavior.

miR-379-5p has potential as a biomarker for AML by regulating cell proliferation and apoptosis through the targeting of YBX1.