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A self-amplifying NO-H2S loop mediates melatonin-induced CBF-responsive pathway and cold tolerance in watermelon
Yanliang Guo1, Jiayue Li1, Lingling Liu1
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
The Plant Journal : for Cell and Molecular Biology
|February 24, 2025
Summary
Melatonin enhances plant cold tolerance by activating the CBF pathway. This involves nitric oxide (NO) and hydrogen sulfide (H2S) signaling, mediated by ClNR1 and ClLCD genes, creating a positive regulatory loop.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Melatonin is crucial for plant cold stress tolerance.
- The exact molecular mechanisms underlying melatonin's role in cold tolerance are not fully understood.
Purpose of the Study:
- To elucidate the role of melatonin in enhancing cold tolerance in watermelon.
- To investigate the involvement of nitric oxide (NO) and hydrogen sulfide (H2S) signaling pathways in melatonin-mediated cold response.
Main Methods:
- Overexpression and knockout of melatonin biosynthetic gene ClCOMT1.
- Application of exogenous melatonin, NO donor (SNP), and H2S donor (NaHS).
- Analysis of C-repeat binding factor (CBF)-responsive pathway, NO and H2S levels, and expression of ClNR1 and ClLCD genes.
Main Results:
- Overexpressing ClCOMT1 or applying melatonin enhanced cold tolerance by activating the CBF pathway.
- Melatonin treatment increased NO and H2S levels, with upregulation of ClNR1 and ClLCD.
- SNP and NaHS application mimicked melatonin's effects, while ClNR1 or ClLCD knockout abolished these responses.
Conclusions:
- A positive regulatory loop between ClNR1-mediated NO and ClLCD-mediated H2S is essential for melatonin-induced cold tolerance.
- Melatonin utilizes NO and H2S signaling to enhance plant cold stress resilience.
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