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Updated: May 26, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Genomic insights and phylogenetics of the mitochondrial genome of Cryptocarya
Jiepeng Huang1, Di Zhang2, Shiting Yang1
1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education) and University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi Normal University, Guilin, Guangxi 541004, China.
Abstract:
The tropical genus Cryptocarya is known for its valuable timber and the constituents in these trees show potential for medicinal properties. However, the phylogenetic relationships among species in Asia remain unclear. Here, we report the first mitochondrial genome for Cryptocarya kwangtungensis, consisting of 758,020 bp, including 43 protein-coding genes, 23 tRNA genes and three rRNA genes, with 234 simple sequence repeats, and 1275 dispersed repeats, 35 homologous DNA fragments between the mitogenome and the plastome. Comparative genomic analysis indicated frequent recombination events among the sequences of five magnoliids mitogenomes and only five conserved clustered genes. Further phylogenetic analyses based on 91 mitochondrial regions and nuclear ribosomal cistron sequences of 21 species compound three well-resolved congruent groups for the Cryptocarya species in Asia, both of which support the genus divide into three clades, suggesting that the mitogenome sequence can provide strongly supported relationships within the genus in the family Lauraceae.
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