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Related Concept Videos

Oogenesis01:22

Oogenesis

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Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
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Related Experiment Video

Updated: May 26, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
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Maternal PRDM10 activates essential genes for oocyte-to-embryo transition.

Michelle K Y Seah1,2, Brenda Y Han1, Yan Huang3

  • 1Institute of Molecular and Cell Biology (IMCB), Agency for Science Technology and Research (A*STAR), Singapore, Singapore.

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|February 24, 2025
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Summary

Maternal PRDM10 is crucial for early embryonic development. Its absence causes oocyte-to-embryo transition failure, highlighting PRDM10

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Area of Science:

  • Developmental Biology
  • Genetics
  • Cell Biology

Background:

  • PR/SET domain-containing (PRDM) proteins regulate mammalian development.
  • Specific PRDM members are vital for germ cell development and stem cell maintenance.

Purpose of the Study:

  • To identify the first PRDM family member with a maternal effect.
  • To investigate the role of maternal PRDM10 in early embryogenesis.

Main Methods:

  • Maternal effect analysis in Prdm10-deficient models.
  • Transcriptomic analysis of oocytes and early embryos.
  • Genome-wide chromatin-binding assays.
  • Investigation of Septin11 expression and function.

Main Results:

  • Absence of maternal PRDM10 leads to complete arrest at the 2-cell stage.
  • Defects in PRDM10 target gene transcript accumulation in oocytes and zygotes.
  • Identification of Septin11 as a key PRDM10 target essential for polar body extrusion.
  • Maternal PRDM10 is required for Septin-complex assembly.

Conclusions:

  • Maternal PRDM10 is essential for oocyte-to-embryo transition.
  • The maternal Septin-complex is critical for early embryonic development.
  • This regulatory axis involving PRDM10 and Septin11 may be conserved in human female germ cells.