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Updated: Aug 3, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Preparation of Polydeoxyribonucleotide Nanoliposomes and their Applicability to Cosmetic Formulations
Yifan He1,2,3, Li Xu3, Xiaojing Pei3
1School of Biomedical Science and Engineering, South China University of Technology, Guangzhou, 510641, P.R. China.
Introduction:
The use of polydeoxyribonucleotide (PDRN) in promoting tissue repair and anti-aging has been hindered by several challenges, including its large molecular weight, susceptibility to decomposition, low bioavailability, and poor stability and skin permeability. Liposome formulation technology has emerged as a promising method in cosmetics, enhancing the penetration and protection of active ingredients.
Method:
In this study, a PDRN-loaded nanoliposomal (PDRN-NL) formulation, which exhibited an average particle size of 125 ± 1 nm, a polydispersity index (PDI) of 0.12 ± 0.02, a zeta potential of -52.6 ± 0.8 mV, and an encapsulation efficiency of 81.3%. The stability of the PDRN-NL and its formulations was assessed using the Turbiscan Lab stability analyzer, which predicted a shelf life of up to three years. Furthermore, the in vitro permeability of the PDRN-NL was evaluated using the Franz diffusion cell method.
Results:
Results indicated that the cumulative skin permeation of PDRN-NL over 24 hours was 1.22 times higher than that of free PDRN, with skin retention of PDRN-NL being 1.40 times greater. The in vitro release studies demonstrated that liposomal encapsulation not only enhanced permeability but also provided a sustained-release effect and improved stability of PDRN.
Conclusion:
Overall, the properties of liposome-encapsulated PDRN were significantly enhanced, presenting a novel solution for the incorporation of PDRN in the development of skincare products.
