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The time course of in vivo cellular responses to LNPs
David Loughrey1, Kalina Paunovska1, Elisa Schrader Echeverri1
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA. david.loughrey@gmail.com.
Abstract:
Cells actively respond to drug delivery systems. However, the time course of cellular responses to lipid nanoparticles (LNPs) remains unclear. Here we characterized the transcriptomic response to LNPs carrying mRNA at different timepoints in vivo. Exposure to LNPs altered the expression of signaling pathways including endocytosis and lysosomal pathways as soon as one hour after administration. These pathways returned to their baseline state by 24 hours. Our data are consistent with the hypothesis that cells actively yet transiently respond to LNPs.
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