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  5. Agricultural, Veterinary And Food Sciences
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  11. Dual-mode Immunochromatographic Test Strips Based On Aggregation-induced Luminescence Nanoprobes For The Detection Of Zen In Corn

Dual-mode immunochromatographic test strips based on aggregation-induced luminescence nanoprobes for the detection of ZEN in corn

Haitao Xu1,2,3, Chunlei Yu1,2,3, Keyun Ren1,2,3

  • 1School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. yqqing@sdut.edu.cn.

Analytical Methods : Advancing Methods and Applications
|February 25, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

A new dual-mode test strip using aggregation-induced emission polymer microspheres enables rapid detection of zearalenone (ZEN). This method overcomes limitations of traditional fluorescent detection, offering high sensitivity and reliability for zearalenone analysis.

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Zearalenone (ZEN) is a widespread mycotoxin with reproductive toxicity, hepatotoxicity, and carcinogenicity.
  • Current rapid ZEN detection methods are hindered by the aggregation-caused quenching (ACQ) effect in fluorescent materials.
  • There is a critical need for novel, sensitive, and reliable ZEN detection techniques.

Purpose of the Study:

  • To develop a novel dual-mode (colorimetric/fluorescent) immunochromatographic test strip for sensitive zearalenone detection.
  • To synthesize and characterize aggregation-induced emission polymer microspheres (AIEPMs) for enhanced detection capabilities.
  • To overcome the limitations of ACQ effects in existing fluorescent detection methods.

Main Methods:

  • Synthesis of AIE polymer microspheres (AIEPMs) incorporating aggregation-induced emission (AIE) dyes.

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  • Preparation of immunoprobes by coupling AIEPMs with antibodies against ZEN.
  • Development of an immunochromatographic test strip (ICA) employing the AIEPM-based immunoprobes for dual-mode detection.
  • Optimization of assay parameters and validation using standard solutions and sample analysis.

    • Main Results:

    • The AIEPM-based immunochromatographic test strip achieved a visual limit of detection (vLOD) of 0.625 ng mL -1 for the colorimetric mode.
    • The fluorescent mode exhibited a significantly lower quantitative limit of detection (qLOD) of 0.039 ng mL -1 .
    • The method demonstrated excellent accuracy with average recoveries ranging from 97.60% to 102.46% and high reproducibility with coefficients of variation (CV) between 2.10% and 10.70%.

    Conclusions:

    • The developed AIEPM-based immunochromatographic test strip offers a sensitive and reliable method for zearalenone detection.
    • The dual-mode capability provides flexibility and enhanced detection limits, overcoming ACQ limitations.
    • This AIEPM-ICA method shows significant potential for rapid, accurate, and on-site screening of zearalenone contamination.