The Impact of Ozone on Periodontal Cell Line Viability and Function

Area of Science:

• Periodontology
• Biomedical Engineering
• Dental Therapeutics

Background:

• Periodontal diseases are chronic inflammatory conditions leading to tissue destruction.
• Standard treatments like scaling and root planing face challenges due to antibiotic resistance.
• Ozone therapy is emerging as a potential adjunctive treatment for periodontal disease.

Purpose of the Study:

• To review the multifaceted mechanisms of ozone therapy in periodontal disease.
• To explore the antimicrobial, immunomodulatory, and regenerative effects of ozone.
• To discuss the clinical applications and future directions of ozone therapy in periodontology.

Main Methods:

• Review of existing literature on ozone therapy in periodontal disease.
• Analysis of ozone's mechanisms of action, including antimicrobial effects on biofilms.
• Examination of ozone's impact on host cells and tissue repair pathways.
• Discussion of in vitro studies on periodontal cell lines.

Main Results:

• Ozone therapy exhibits selective antimicrobial action against periodontal pathogens and disrupts biofilms.
• It modulates host immune responses and stimulates tissue repair by activating Nrf2 and MAPK pathways.
• Ozone therapy upregulates growth factors and extracellular matrix proteins, crucial for regeneration.
• In vitro studies show positive effects on periodontal cell viability, proliferation, and differentiation.

Conclusions:

• Ozone therapy offers dual antimicrobial and regenerative capabilities as an adjunctive periodontal treatment.
• It demonstrates significant clinical benefits when combined with periodontal regenerative techniques.
• Standardization of dosages, delivery systems, and long-term studies are necessary for clinical integration.
• Optimized protocols and evidence-based guidelines are essential to maximize ozone therapy's potential in enhancing periodontal health.