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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

A Ratiometric Fluorescence Nano pH Biosensor for Live-Cell Imaging Using Cerasome.

Zhongqiao Zhang¹, Xiaoshan Luo², Xuanbo Wang²

¹School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

|February 25, 2025

Summary

Researchers developed a novel nanoscale pH sensor using silica-coated liposomes (cerasomes). This ultrastable, biocompatible sensor precisely monitors intracellular pH for disease diagnosis and cellular biology research.

Keywords:

cerasome live cells nanoparticles pH sensor ratiometric fluorescence

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Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator

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Published on: April 5, 2017

Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Accurate pH monitoring is crucial for understanding cellular processes and diagnosing diseases.
Existing pH sensors often face limitations in stability, biocompatibility, and precision.
Developing advanced nanoscale platforms is essential for real-time intracellular measurements.

Purpose of the Study:

To develop a robust, biocompatible, and ultrastable ratiometric fluorescence nano pH sensor.
To integrate a pH-sensitive dye within a novel nanoparticle structure for enhanced performance.
To enable precise real-time intracellular pH monitoring for biomedical applications.

Main Methods:

Fabrication of silica-coated liposome nanoparticles (cerasomes) with an average size of 138.4 nm.
Incorporation of the pH-sensitive dye pyranine within the cerasome structure.
Characterization of the sensor's ratiometric fluorescence properties, photostability, sensitivity, and morphological stability.

Main Results:

The cerasome-based nano pH sensor demonstrated enhanced photostability, sensitivity, and biocompatibility.
A linear detection range of pH 6.25-8.5 was achieved with minimal photobleaching and quenching.
The nanoparticles exhibited high morphological stability, suitable for intracellular pH measurements.

Conclusions:

The developed ratiometric fluorescence nano pH sensor offers a versatile and promising tool for biomedical research.
This platform facilitates precise real-time intracellular pH monitoring, aiding cellular biology and disease diagnosis.
The ultrastable and biocompatible nature of the cerasome sensor opens avenues for therapeutic monitoring.