Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

How does national power promote the improvement of the application level of electronic medical record.

Frontiers in public health·2026

Same author

Development and preliminary clinical validation of A29L mAb-based ELISA and lateral flow immunoassays for monkeypox virus detection.

Microbiology spectrum·2026

Same author

Efficacy of remimazolam besylate versus propofol on hemodynamics during anesthetic induction in elderly patients undergoing bronchoscopy: a randomized controlled trial.

BMC anesthesiology·2026

Same author

Application of an artificial-intelligence-based transesophageal echocardiography simulation system in residency training.

Frontiers in surgery·2026

Same author

Peripheral immune cells and glycation indices as potential diagnostic biomarkers in amyotrophic lateral sclerosis.

Experimental biology and medicine (Maywood, N.J.)·2026

Same author

Advancing Personalized Medicine through Vessel-Supported Patient-Established Lung Tumor Organoids: A Microfluidic Approach.

ACS biomaterials science & engineering·2026

Search research articles

Related Experiment Video

Updated: May 26, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

The Latest Advances in Microfluidic DLD Cell Sorting Technology: The Optimization of Channel Design.

Dan Fan¹, Yi Liu¹, Yaling Liu^2,3

¹School of Engineering, Dali University, Dali 671003, China.

|February 25, 2025

Summary

Deterministic lateral displacement (DLD) is a microfluidic technique for cell sorting. This review covers recent channel design optimizations for improved cell separation efficiency and accuracy in biomedical research.

Keywords:

DLD cell sorting channel design machine learning microfluidics

More Related Videos

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Published on: November 10, 2014

Related Experiment Videos

Last Updated: May 26, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Published on: November 10, 2014

Area of Science:

Microfluidics
Biotechnology
Cell Biology

Background:

Cell sorting is vital for medical and biological research.
Deterministic lateral displacement (DLD) is a passive microfluidic technique widely used for cell separation.
Optimizing DLD channel design is key to enhancing cell sorting performance.

Purpose of the Study:

To review the latest advancements in microfluidic deterministic lateral displacement (DLD) channel design for cell sorting.
To highlight innovations, applications in biomedical fields, and integration with machine learning.
To discuss current challenges and future prospects in DLD channel design optimization.

Main Methods:

Overview of microfluidic DLD channel design elements.
Analysis of factors influencing separation efficiency and accuracy (channel geometry, fluid dynamics, cell-surface interactions).
Review of recent innovations and progress in DLD channel design.

Main Results:

Recent innovations focus on optimizing channel geometry and fluid dynamics for improved cell separation.
Integration with machine learning shows promise for advanced DLD control and analysis.
Key factors affecting separation include pillar design, flow rate, and cell properties.

Conclusions:

Optimized DLD channel designs are crucial for high-efficiency and high-accuracy cell sorting in various biomedical applications.
Further research is needed to address current challenges and fully exploit the potential of DLD technology.
Future directions include advanced computational modeling and integration with other sorting techniques.