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Bisphenol A Release from Fiber-Reinforced vs. Conventional Stainless-Steel Fixed Retainers: An In Vitro Study
Efthimia Tsoukala1, Niki Maragou2, Andriani-Paraskevi Antonelaki2
1Department of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Journal of Functional Biomaterials
|February 25, 2025
Summary
Bisphenol A (BPA) release from fiberglass and stainless-steel orthodontic retainers did not significantly differ in the first month. Highest BPA concentrations were observed at 30 days for all tested retainer types.
Area of Science:
- Orthodontic Materials Science
- Biomaterials Research
- Analytical Chemistry
Background:
- Bisphenol A (BPA) is a chemical compound used in some dental materials.
- Fiberglass-reinforced composite resins are increasingly used as orthodontic retainers.
- Understanding potential BPA leaching from these materials is crucial for patient safety.
Purpose of the Study:
- To quantify in vitro Bisphenol A (BPA) release from two common fiberglass lingual retainers.
- To compare BPA release from fiberglass retainers with a conventional stainless-steel orthodontic retainer.
Main Methods:
- Three retainer types (Ribbond, EverStick Ortho, Wildcut wire) were tested on extracted teeth.
- BPA release was measured using liquid chromatography-tandem mass spectrometry at multiple time points up to 30 days.
- Statistical analysis involved a two-way mixed, repeated-measures ANOVA.
Main Results:
- Higher BPA levels were detected from polyethylene and glass fiber retainers compared to the stainless-steel control.
- No statistically significant differences in BPA release were found between the retainer systems over the 30-day period.
- The highest BPA concentrations for all groups were observed at the 1-month measurement point.
Conclusions:
- In vitro BPA release during the initial month of use did not significantly vary among the tested fiberglass and stainless-steel orthodontic retainers.
- BPA concentrations peaked at 1 month, suggesting potential accumulation or degradation over time.
- Further research may be warranted to assess long-term BPA release and clinical implications.

