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A method for preparing and handling cross-sections from early chick embryos for scanning electron microscopy.
Journal of Microscopy
|July 1, 1979
Summary
Researchers developed a new method for preparing early chick embryo cross-sections for scanning electron microscopy. This technique simplifies processing multiple sections from single embryos, improving reliability and reducing tissue handling challenges.
Area of Science:
- Developmental Biology
- Microscopy Techniques
Background:
- Scanning electron microscopy (SEM) requires meticulously prepared biological samples.
- Processing delicate embryonic tissues, especially multiple cross-sections, presents significant technical challenges.
Purpose of the Study:
- To describe a novel and reliable method for processing multiple cross-sections from early chick embryos for SEM.
- To overcome the difficulties associated with handling brittle, dried embryonic tissues.
Main Methods:
- Early chick embryos were sectioned at desired regions.
- Sections were mounted onto coverslips using Duco cement.
- Samples underwent critical point drying using either Freon 13 or liquid carbon dioxide (CO2).
Main Results:
- The described method allows for the preparation of multiple cross-sections from a single embryo.
- The technique successfully avoids direct handling of tissues after the critical point drying stage.
Conclusions:
- This method offers a dependable approach for preparing early chick embryo cross-sections for SEM.
- The technique enhances the ease and reliability of SEM sample preparation for developmental studies.