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Updated: May 26, 2025

Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using 129Xe MRI

Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using ¹²⁹Xe MRI

Published on: November 21, 2023

Functional Lung Imaging Identifies Peripheral Ventilation Changes in ꞵ-ENaC Mice.

Nicole Reyne^1,2,3, Ronan Smith^1,2,3, Patricia Cmielewski^1,2,3

¹Robinson Research Institute, University of Adelaide, Adelaide, South Australia, Australia.

Respirology (Carlton, Vic.)

|February 25, 2025

Summary

This summary is machine-generated.

The beta-epithelial sodium channel transgenic (β-ENaC-Tg) mouse model exhibits significant lung function impairments, including increased ventilation defects and parenchymal damage. X-ray velocimetry and flexiVent analyses reveal critical insights into muco-obstructive lung disease progression.

Keywords:

animal models cystic fibrosis lung disease lung function muco‐obstructive x‐ray velocimetry

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Area of Science:

Pulmonary physiology and disease modeling.
Respiratory system mechanics.
Medical imaging and diagnostics.

Background:

Beta-epithelial sodium channel transgenic (β-ENaC-Tg) mice model muco-obstructive lung disease and emphysema.
These mice exhibit impaired mucociliary clearance, mucus obstruction, chronic inflammation, and lung structure damage.
Altered lung function is a hallmark of this disease model.

Purpose of the Study:

To conduct a comprehensive analysis of lung function and mechanics in adult β-ENaC-Tg mice.
To evaluate the utility of X-ray velocimetry (XV) in assessing ventilation defects.
To compare lung mechanics using flexiVent in β-ENaC-Tg and wild-type mice.

Main Methods:

X-ray velocimetry (XV) scans were performed on adult β-ENaC-Tg and wild-type littermates.
Lung function assessments were conducted using a flexiVent system for comparative lung mechanics.
Spatial analysis of ventilation maps was employed to assess regional lung function.

Main Results:

XV imaging revealed elevated ventilation defect percentage, mean specific ventilation, and ventilation heterogeneity in β-ENaC-Tg mice.
Spatial analysis showed increased ventilation variability and under-ventilated areas in peripheral lung regions.
FlexiVent analysis demonstrated increased lung compliance, inspiratory capacity, and hysteresivity, with reduced tissue elastance in β-ENaC-Tg mice, indicating loss of parenchymal integrity.

Conclusions:

This study provides a comprehensive lung function and mechanics analysis of the β-ENaC-Tg mouse model.
X-ray velocimetry is highlighted as a valuable tool for evaluating ventilation defects in this model.
Findings underscore the significant lung parenchymal and functional alterations in β-ENaC-Tg mice, relevant for muco-obstructive lung disease research.