Molecular engineering of functional DNA molecules toward point-of-care diagnostic devices

The pursuit of rapid, sensitive, and specific diagnostic methodologies is imperative across diverse applications, including the detection of pathogens and disease biomarkers, food safety testing and environmental monitoring. Point-of-care testing (POCT) is characterized by its portability, ease of use, rapidity, and affordability, emerging as an attractive alternative for traditional diagnostics. Over recent years, the incorporation of functional DNA (fDNA) into POC diagnostic devices has emerged as a groundbreaking advancement, significantly enhancing sensitivity, specificity, and user-friendliness. In this review, we explore the innovative applications of fDNA in POC devices, highlighting its potential to revolutionize diagnostics by providing rapid, portable, and precise solutions. We discuss the unique advantages of fDNA, including its stability in complex biological matrices and its ability to recognize a wide range of targets. Furthermore, we explore the potential synergy between fDNA and cutting-edge technologies, such as nanotechnology and artificial intelligence (AI), to forge a path toward more personalized and accessible healthcare solutions. Despite significant progress, challenges remain in translating these innovations from the bench to the clinic. This review aims to provide a comprehensive overview of the current status of fDNA-based POCT devices and future directions for their development, emphasizing their critical role in meeting the global demand for accessible, efficient, and precise diagnostic solutions.