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Published on: December 16, 2022
Protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice
Jiameng Sun1, Brian J DeBosch2
1Department of Pediatrics, Washington University School of Medicine in St. Louis, St. Louis, MO 63110, USA; Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Abstract:
To investigate the role of hepatocyte genes in fasting metabolism, it is essential to analyze their expression across the entire 24-h circadian cycle. Here, we present a protocol to evaluate fasting metabolism and its relationship to the core circadian clock in mice. We describe steps for time-course fasting/feeding experiment setup and performing staggered fasting and refeeding for 24 h. We then detail procedures for evaluating oxidative substrate selection and rescuing defective oxidative metabolism through FGF21 and CPI-613 injections. For complete details on the use and execution of this protocol, please refer to Sun et al.1.
