Molecular and Seroprevalence of Mycoplasma gallisepticum in Turkeys in Sylhet District of Bangladesh

Jahid Hasan Tipu ^1,2, Rijon Miah ², Obaidul Islam ³

¹Centre for International Health, Department of Global Public Health and Primary Care, Faculty of Medicine, University of Bergen, Bergen, Norway.
²Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.
³Laboratory of Veterinary Epidemiology, College of Veterinary Medicine, Chungbuk National University, Chungbuk, South Korea.
⁴Department of Veterinary Sciences, Chonnam National University, Gwangju, South Korea.
⁵School of Public Health, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
⁶Laboratory of Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University, Chungbuk, South Korea.
⁷School of Life Sciences, University of Warwick, Coventry, UK.
⁸Department of Epidemiology and Public Health, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.
⁹Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.

⁰Centre for International Health, Department of Global Public Health and Primary Care, Faculty of Medicine, University of Bergen, Bergen, Norway.

Veterinary Medicine and Science +

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February 25, 2025

View abstract on PubMed

Summary

Mycoplasma gallisepticum (MG) infection is prevalent in Bangladesh

Area Of Science

Veterinary Science
Microbiology
Animal Health

Background

Mycoplasma gallisepticum (MG) causes significant economic losses in Bangladesh's poultry sector.
Accurate diagnosis and understanding risk factors are crucial for controlling MG outbreaks.

Purpose Of The Study

To determine the prevalence of MG in turkeys in Sylhet, Bangladesh.
To evaluate the diagnostic accuracy of Serum Plate Agglutination (SPA), ELISA, and PCR.
To identify risk factors associated with MG infection in turkeys.

Main Methods

Serum plate agglutination (SPA), ELISA, and PCR were used to test 250 blood and 250 tracheal swab samples from turkeys.
Samples were collected from 25 farms across three sub-districts of Sylhet from December 2019 to November 2020.
PCR targeted the 16S rRNA gene of MG, with SPA and ELISA sensitivity/specificity evaluated against PCR.

Main Results

Overall MG prevalence was 35.2% (SPA), 29.2% (ELISA), and 25.6% (PCR).
Higher infection rates were observed in younger turkeys (0-4 months), during winter, and in female turkeys.
Beanibazar sub-district showed the highest prevalence; SPA and ELISA demonstrated high sensitivity (100%) with varying specificity.

Conclusions

MG infection is a significant concern for turkey farming in Sylhet, Bangladesh.
PCR is a reliable diagnostic tool, and SPA/ELISA show potential for field diagnosis.
Findings support targeted control strategies to mitigate economic losses in the poultry industry.

Keywords:

16S rRNA gene Diagnostic tests Mycoplasma gallisepticum (MG) Sylhet poultry sensitivity specificity