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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
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Rotavirus Infection in Children Suffering from Diarrhea in Patna, Bihar Region, India: A Hospital-Based Study

Neetesh Jindal1, Ashish Kumar1,2, Ashiq Hussain Bhat3

  • 1Department of Virology, ICMR-Rajendra Memorial Research Institute of Medical Sciences, Patna, India.

The American Journal of Tropical Medicine and Hygiene
|February 25, 2025
PubMed

Insights

Rotavirus (RV) infection prevalence in Bihar, India, was 10%, with the highest rates in children aged 0-11 years. Genetic analysis revealed distinct regional RV strains, underscoring the need for targeted vaccination and surveillance.

Area of Science:

  • Medical Microbiology
  • Virology
  • Epidemiology

Background:

  • Rotavirus (RV) is a major cause of severe gastroenteritis in young children globally.
  • Limited data exists on RV prevalence and genetic diversity in Bihar, India.

Purpose of the Study:

  • To investigate the prevalence and molecular/epidemiological patterns of RV infections in Bihar.
  • To assess the genetic diversity of RV strains in the region.

Main Methods:

  • Retrospective analysis of 1,820 stool samples from suspected RV cases.
  • Enzyme immunoassays for antigen detection, confirmed by real-time PCR (VP4, VP6, VP7 genes).
  • Phylogenetic analysis for genetic diversity assessment.

Main Results:

  • A 10% RV positivity rate was observed, with 5% equivocal results.
  • Highest prevalence in the 6-11 years age group (72 cases), followed by 0-5 years (62 cases).
  • Phylogenetic analysis identified distinct regional RV clusters and genetic variability compared to other Indian regions.

Conclusions:

  • This study provides crucial baseline data on RV prevalence and genetic diversity in Bihar.
  • Highlights the need for vaccination and enhanced surveillance, especially for at-risk young children.
  • Observed genetic diversity emphasizes the importance of continuous nationwide RV surveillance.

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