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Unraveling biochemical differences in the membrane of functional RBCs and elliptocytes using vortex beam-based
Panchanil Sarmah1, Cheviri Ghanashyam1, Ruchee Khanna2
1Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal 576104, India.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|February 25, 2025
Summary
This study uses single-cell Raman spectroscopy to reveal biochemical changes in red blood cell membranes of elliptocytosis, offering new insights into hereditary elliptocytosis.
Area of Science:
- Biophysics
- Cell Biology
- Spectroscopy
Background:
- Hereditary elliptocytosis involves red blood cell membrane abnormalities.
- Current methods like ektacytometry and EMA binding tests cannot study metabolically active elliptocytes.
- Understanding in-vitro induced elliptocytosis can model hereditary conditions.
Purpose of the Study:
- To investigate biochemical modifications in the membrane and cytoskeleton of elliptocytes.
- To utilize single-cell Raman spectroscopy for studying functional cells.
- To overcome limitations of existing methods in assessing elliptocytes.
Main Methods:
- Employed single-cell Raman spectroscopy.
- Utilized a vortex beam to probe the red blood cell membrane.
- Reduced spectral interference from hemoglobin for clearer analysis.
Main Results:
- Raman spectral variations indicated changes in proteins, lipids, and their interactions within elliptocytes.
- Observed diversity in membrane components among individuals.
- Noted variations in membrane-hemoglobin interactions.
Conclusions:
- Single-cell Raman spectroscopy is effective for analyzing biochemical changes in elliptocytes.
- The findings provide insights into the membrane alterations in elliptocytosis.
- The study highlights the heterogeneity of membrane components and interactions.
