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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Near-infrared fluorescence imaging with an MET-targeting probe for biopsy site selection in patients with oral

Jingbo Wang1, Xuemin Shen2, Qifan Ma1

  • 1Department of Radiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Cell Reports. Medicine
|February 25, 2025
PubMed
Summary

Near-infrared fluorescence imaging (NIRFI) with a MET-targeted probe improves oral potentially malignant disorder (OPMD) biopsy accuracy. This advanced technique significantly reduces missed high-grade dysplasia or cancer detection compared to conventional methods.

Keywords:
4-NQO-induced OSCC modelbiopsy site selectionfluorescence-guided biopsymesenchymal-epithelial transition factormolecular imagingnear-infrared targeted fluorescent imagingoral potentially malignant disordersoral squamous cell carcinomatopical application

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Area of Science:

  • Oncology
  • Medical Imaging
  • Biotechnology

Background:

  • Accurate detection of malignant transformation in oral potentially malignant disorders (OPMDs) is critical for effective patient management and treatment.
  • Conventional oral examination (COE) can have limitations in precisely identifying areas requiring biopsy.

Purpose of the Study:

  • To evaluate the diagnostic potential of a mesenchymal-epithelial transition factor (MET)-targeted near-infrared fluorescence imaging (NIRFI) probe (cMBP-ICG) for biopsy site selection in OPMDs.
  • To compare the accuracy of NIRFI-assisted biopsy with COE-based biopsy in detecting high-grade dysplasia (HGD) or squamous cell carcinoma (SCC).

Main Methods:

  • Development and application of a MET-binding peptide-indocyanine green (cMBP-ICG) probe for NIRFI.
  • Preclinical evaluation and a clinical trial involving 50 patients with OPMDs.
  • Comparison of diagnostic accuracy between NIRFI-assisted biopsy and COE-based biopsy.

Main Results:

  • Preclinical studies showed superior accuracy of NIRFI-assisted biopsy in detecting HGD/SCC and reducing missed diagnoses.
  • The clinical trial demonstrated significantly higher diagnostic accuracy for NIRFI-assisted biopsy (91%) compared to COE-based biopsy (72%, p = 0.0005).

Conclusions:

  • NIRFI using the cMBP-ICG probe significantly enhances diagnostic precision for OPMDs.
  • This technology supports earlier detection and more accurate treatment interventions for OPMD patients.
  • NIRFI represents a promising tool for improving biopsy site selection in OPMDs.