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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Exploring Single-Probe Single-Cell Mass Spectrometry: Current Trends and Future Directions.

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Single-probe single-cell mass spectrometry (SCMS) offers miniaturized, ambient metabolomic profiling. This technique enables direct analysis of cells in native environments for diverse microscale bioanalysis applications.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Biophysics

Background:

  • Single-cell mass spectrometry (SCMS) is an innovative analytical technique.
  • It utilizes a miniaturized, multifunctional device for direct coupling to mass spectrometers.
  • SCMS is an ambient technique employing microscale sampling and nanoelectrospray ionization (nanoESI).

Purpose of the Study:

  • To review recent advancements in single-probe-based techniques.
  • To highlight applications of SCMS in microscale bioanalysis.
  • To emphasize the potential of SCMS in advancing mass spectrometry methodologies.

Main Methods:

  • Ambient technique leveraging microscale sampling.
  • Direct coupling to mass spectrometers.
  • Nanoelectrospray ionization (nanoESI) for sample introduction.

Main Results:

  • Enables analysis of cells in native environments without extensive sample preparation.
  • Facilitates diverse applications including single-cell metabolomics and MS imaging.
  • Allows quantification of target molecules in single cells and investigation of extracellular molecules.

Conclusions:

  • SCMS is a versatile technique for microscale bioanalysis.
  • Advancements in SCMS offer significant potential for mass spectrometry.
  • The technique supports various applications from single-cell to tissue analysis.