Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics

Area of Science:

• Biochemistry and Molecular Biology
• Analytical Chemistry

Background:

• Mass spectrometry-based proteomics has a 35-year history.
• Recent years have seen accelerated progress across all subfields of proteomics.

Purpose of the Study:

• To review recent advances in select areas of bottom-up proteomics.
• Focus on developments within the last two years.

Main Methods:

• Review of recent literature and technological advancements.
• Focus on specific subfields of bottom-up proteomics.

Main Results:

• Highlighting progress in high-throughput proteomics approaches.
• Discussing machine learning applications in proteomics data analysis.
• Summarizing advances in drug discovery, glycoproteomics, extracellular vesicle proteomics, and structural proteomics.

Conclusions:

• The field of mass spectrometry proteomics is rapidly evolving.
• Recent innovations are enhancing capabilities in various applications and subfields.