Characterization of parasite antigens from human hydatid cyst fluid by SDS-PAGE and IEF

Combining high resolution power of SDS-PAGE and IEF with the specific immunological recognition of a human antiserum directed against Echinococcus granulosus antigens, we could identify, in 4 hydatid cystic fluids of human origin, 4 antigens with a molecular weight in the range 32-13 KD, and an antigen of 200 KD which, in reducing conditions, gave 2 bands of 67 and 52 KD. In addition, mainly in one of the cystic fluids, there were at least another 4 specific non-reducible bands with a molecular weight ranging from 80 to 40 KD. Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8.