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Updated: May 25, 2025
Dissection of Hippocampal Dentate Gyrus from Adult Mouse
Published on: November 17, 2009
Identification of a novel transcript of mouse Sdha
María J Esteban-Amo1,2, Amaia Telleria1,2, Dino Gobelli1,2
1Department of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of Valladolid, 47005, Valladolid, Spain.
Objective:
This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA.
Results:
We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies.
