DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid-Based Cytology

Area of Science:

• Cytopathology
• Flow Cytometry
• Biomedical Analysis

Background:

• DNA flow cytometry with LC-1000 analyzes cellular DNA content and proliferation index (CPIx).
• Body cavity fluid cytology requires reliable cell preservation solutions for accurate analysis.
• Evaluating new preservation solutions like CelVerse (CeV) against established ones like Cellprep (CP) is crucial.

Purpose of the Study:

• To evaluate the performance of body cavity fluid cytology using the LC-1000 system.
• To compare the efficacy of CelVerse (CeV) as a cell preservation solution against Cellprep (CP).
• To assess the impact of different preservation solutions on the cell proliferation index (CPIx) and diagnostic accuracy.

Main Methods:

• Compared 43 body cavity fluid samples in CeV and 59 in CP using LC-1000.
• Analyzed cell proliferation index (CPIx) in malignant and benign cases for both CeV and CP groups.
• Assessed the influence of SubG1% on diagnostic performance, measured by area under the curve (AUC).

Main Results:

• Both CeV and CP showed significant differences in CPIx between malignant and benign cases (p < 0.001).
• CelVerse (CeV) demonstrated a higher AUC (0.811) compared to Cellprep (CP) (0.776).
• The difference in SubG1% significantly impacted CP's AUC, while CeV results remained consistent.

Conclusions:

• Cell proliferation index (CPIx) using LC-1000 in CeV or CP samples is a viable objective indicator for body cavity fluid cytology.
• CelVerse (CeV) offers more consistent results in body cavity fluid cytology, unaffected by SubG1% variations.
• CeV shows potential as a superior preservation solution for LC-1000 based body cavity fluid cytology.