Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

43.3K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.3K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

61.7K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
61.7K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Antibiotic Selection00:57

Antibiotic Selection

52.2K
Overview
52.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact on daptomycin resistance using combination of daptomycin plus ceftaroline in daptomycin-susceptible and -resistant <i>Staphylococcus aureus</i>.

FEMS microbes·2026
Same author

Virulence Phenotypes Differentiate Persistent vs. Resolving Isolates of Human <i>Staphylococcus aureus</i> Bacteremia.

Antibiotics (Basel, Switzerland)·2026
Same author

Optimizing phage-antibiotic combinations: impact of administration order against daptomycin non-susceptible (DNS) MRSA clinical isolates.

Antimicrobial agents and chemotherapy·2025
Same author

Prophage-encoded virulence factor, Gp05, contributes to endothelial cell dysfunction and immune evasion to promote persistent methicillin-resistant <i>Staphylococcus aureus</i> endovascular infections.

mBio·2025
Same author

Genetic Correlates of Synergy Mechanisms of Daptomycin Plus Fosfomycin in Daptomycin-Susceptible and -Resistant Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA).

Microorganisms·2025
Same author

Phage-Encoded Virulence Factor, Gp05, Alters Membrane Phospholipids and Reduces Antimicrobial Susceptibility in Methicillin-Resistant Staphylococcus aureus.

The Journal of infectious diseases·2024

Related Experiment Video

Updated: May 25, 2025

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

24.0K

Prophage ϕSA169 Enhances Vancomycin Persistence in Methicillin-Resistant Staphylococcus aureus (MRSA).

Yi Li1, Andrew D Berti2, Wessam Abdelhady1

  • 1The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Antibiotics (Basel, Switzerland)
|February 26, 2025
PubMed
Summary

Prophage ϕSA169 enhances vancomycin (VAN) persistence in methicillin-resistant Staphylococcus aureus (MRSA) by promoting biofilm formation and VAN tolerance across diverse genetic backgrounds, suggesting new therapeutic targets.

Keywords:
MRSA endovascular infectionsprophagevancomycin persistence

More Related Videos

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.8K
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
09:01

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Published on: September 28, 2022

1.9K

Related Experiment Videos

Last Updated: May 25, 2025

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

24.0K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

8.8K
Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
09:01

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Published on: September 28, 2022

1.9K

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Persistent methicillin-resistant Staphylococcus aureus (MRSA) endovascular infections pose a major clinical challenge.
  • Prophages are key genetic factors in S. aureus pathogenicity, but their role in MRSA antibiotic persistence is understudied.
  • Previous research linked prophage ϕSA169 to vancomycin (VAN) persistence in CC45 MRSA.

Purpose of the Study:

  • To investigate the role of prophage ϕSA169 in vancomycin (VAN) persistence across diverse MRSA genetic backgrounds.
  • To determine if ϕSA169 influences VAN susceptibility, biofilm formation, and treatment efficacy in MRSA.
  • To assess the clinical relevance of ϕSA169 in MRSA infections.

Main Methods:

  • Lysogenization of resolving bacteremia (RB) MRSA strains from CC5 and CC30 with ϕSA169.
  • Evaluation of VAN susceptibility, biofilm formation, and VAN treatment efficacy in an experimental infective endocarditis (IE) model.
  • Comparison of ϕSA169 lysogenic strains with their isogenic MRSA parental counterparts.

Main Results:

  • ϕSA169 lysogeny significantly enhanced biofilm formation and survival to VAN exposure in CC5 and CC30 MRSA strains.
  • ϕSA169 reduced VAN effectiveness in the IE model for CC5 MRSA, despite no change in VAN minimum inhibitory concentrations (MICs).
  • Pilot clinical data suggested worse outcomes in patients with MRSA containing ϕSA169-like prophages.

Conclusions:

  • Prophage ϕSA169 promotes VAN persistence across different MRSA clonal backgrounds.
  • Biofilm formation and VAN tolerance are likely mechanisms by which ϕSA169 confers persistence.
  • Targeting prophages may offer novel strategies for combating persistent MRSA infections.