Comparative Transcriptomic Analysis Provides Insight into Spatiotemporal Expression Patterns of Pivotal Genes During...

Area of Science:

• Animal Science
• Genetics
• Developmental Biology

Background:

• Min pigs are known for superior meat quality, but the underlying growth and development patterns are poorly understood.
• Understanding these patterns is crucial for improving meat quality through domestication.

Purpose of the Study:

• To elucidate the relationship between growth patterns and meat quality in Min pigs.
• To identify key genes and genetic mechanisms involved in Min pig development and meat quality.

Main Methods:

• Comparative transcriptomics
• Quantitative real-time PCR (qRT-PCR)
• Selection pressure analysis
• 3D protein structure simulations

Main Results:

• 150 days of age was identified as a critical developmental stage in Min pigs.
• Thirteen key genes showed differential expression between early and late growth stages.
• The CDK2 gene exhibited high expression in hind limb muscles and adipose tissues in later stages, with evidence of strong positive selection pressure due to mutations in non-coding regions.

Conclusions:

• CDK2 plays a vital role in the spatiotemporal development of meat during Min pig domestication.
• This research provides insights into pig growth and offers a foundation for enhancing meat quality traits.